Master's Theses

Department

Biology

Degree Name

Master of Science (MS)

Abstract

A protocol was developed to isolate and identify anaerobic bacteria from surgical specimens. This scheme incorporated specialized media, biochemical tests, a miniaturized biochemical differentiation system, gas liquid chromatography, and the use of a flexible anaerobic glove box. Twenty-five specimens of gallbladder bile were cultured for anaerobes and aerobes. Eleven specimens (44%) were positive, eight (32%) contained at least one anaerobe, and five (20%) grew anaerobes only. Of the 13 anaerobic isolates, Clostridium spp. were most frequently recovered (61.5%). Fourteen miscellaneous surgical specimens also were cultured for anaerobes and aerobes. All 14 specimens were positive, nine (64.3%) grew anaerobes, and three (21.4%) contained anaerobes only. Bacteroides spp. were the most commonly recovered anaerobes (32.1%) from the 28 anaerobic isolates. This study demonstrates the effectiveness of the protocol used for the isolation and identification of anaerobic bacteria.

Advisor

Judith C. Vogt

Date of Award

Fall 1978

Document Type

Thesis - campus only access

Rights

© 1978 Carla Ruder

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