Master's Theses

Document Type

Thesis - campus only access

Date of Award

Fall 1978

Degree Name

Master of Science (MS)

Department

Biology

Advisor

Judith C. Vogt

Abstract

A protocol was developed to isolate and identify anaerobic bacteria from surgical specimens. This scheme incorporated specialized media, biochemical tests, a miniaturized biochemical differentiation system, gas liquid chromatography, and the use of a flexible anaerobic glove box. Twenty-five specimens of gallbladder bile were cultured for anaerobes and aerobes. Eleven specimens (44%) were positive, eight (32%) contained at least one anaerobe, and five (20%) grew anaerobes only. Of the 13 anaerobic isolates, Clostridium spp. were most frequently recovered (61.5%). Fourteen miscellaneous surgical specimens also were cultured for anaerobes and aerobes. All 14 specimens were positive, nine (64.3%) grew anaerobes, and three (21.4%) contained anaerobes only. Bacteroides spp. were the most commonly recovered anaerobes (32.1%) from the 28 anaerobic isolates. This study demonstrates the effectiveness of the protocol used for the isolation and identification of anaerobic bacteria.

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Rights

© 1978 Carla Ruder

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