Master's Theses

Document Type

Thesis - campus only access

Date of Award

Spring 2008

Degree Name

Master of Science (MS)

Department

Biology

Advisor

Greg Farley

Abstract

Non-therapeutic use of antibiotics in the agriculture industry has contributed to the development of antibiotic-resistant bacteria, which subsequently have been detected in air, soil, and surface and ground water by other researchers. From these sources resistant bacteria can be spread throughout ecosystems and potentially enter human populations. Saliva samples were taken from 114 migratory birds at the Fort Hays State University banding site during fall 2006. Each sample was plated on brain heart infusion agar and incubated in order to allow for bacterial growth. Isolated bacterial colonies were then Gram stained and tested for resistance to vancomycin. Differential and selective media were used for preliminary colony screening. Twenty vancomycin-resistant Gram-positive bacterial isolates were putatively identified using partial sequencing of the 16S rRNA gene. Four different bacterial species (Staphylococcus Succinus AF004219, Staphylococcus saprophyticus, Enterococcus gallinarum, and Staphylococcus sp. EU196332) were identified from the twenty vancomycin-resistant isolates collected from nine bird species.

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Rights

© 2008 Jessica Bitner

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