Abstract
This project aims to create CRISPR-CAS9 mutations in the APETELA3 (AP3) gene of the model plant Arabidopsis thaliana. AP3 is a class B gene critical to the petal and stamen development of Arabidopsis flowers. AP3 is defined in a MADS domain, which binds directly to DNA and may be responsible for the expression of the CaRG-box genes. AP3 works in conjunction with PISTILLATA (PI), AGAMOUS (AG), APETALA1 (AP1), and SEPALLATA (SEP) genes to specify the development in the second and third whorls of the flower. While several alleles of AP3 already exist, these alleles are strong alleles that knockout gene function. The advantage of creating weak alleles is that they often reveal gene functions that knockout mutations overlook. Thus, examining the phenotypes caused by weak alleles allows researchers to fully characterize gene function. The CRISPR-CAS9 system can make a variety of mutations including small insertions, deletions, or substitutions. We will specifically choose and examine weak alleles for this project. Isolated DNA from the third generation of AP3-3 has been Sanger sequenced, which confirmed a mutation. A single guanine insertion caused a frame shift leading to a weak allele affecting floral structure.
Faculty Advisor
Dr. Tara Phelps-Durr
Department/Program
Biology
Submission Type
in-person poster
Date
3-31-2025
Rights
Copyright the Author(s)
Recommended Citation
Frans, Hazel and Phelps-Durr, Tara
(2025)
"CRISPR-Induced Mutagenesis of Arabidopsis thaliana Gene APETALA3,"
SACAD: Scholarly Activities: Vol. 2025, Article 68.
Available at:
https://scholars.fhsu.edu/sacad/vol2025/iss2025/68
Included in
Biology Commons, Biotechnology Commons, Developmental Biology Commons, Genetics Commons, Genomics Commons, Molecular Biology Commons, Molecular Genetics Commons, Plant Biology Commons, Plant Breeding and Genetics Commons, Scholarship of Teaching and Learning Commons
