Analyzing Histone Chaperone ANTI-SILENCING FACTOR-1A and HISTONE REPRESSOR A Interactions and Their Roles in Arabidopsis thaliana Development via CRISPR/Cas9 Mutagenesis

Authors

Muyang Xu, Fort Hays State UniversityFollow

Classification

Empirical Undergraduate

Abstract

The ASF1A and HIRA are histone chaperones highly conserved in many organisms. The protein interactions between ASF1a and HIRA and their role in plant development are not understood in Arabidopsis thaliana. Here, we present our attempt to generate mutations at ASF1a and HIRA genes using CRISPR/Cas9 technology and analyze their interactions through computational molecular biology. Our current efforts focus on preliminary sequence analyses, CRISPR/Cas9 system preparation, and transgene delivery.

Faculty Advisor

Dr. Tara Phelps-Durr

Department/Program

KAMS

Submission Type

in-person poster

DOI

10.58809/QRUG3218

Date

4-17-2023

Rights

Copyright the Author(s)

Comments

For questions contact ScholarsRepository@fhsu.edu

Recommended Citation

Xu, Muyang (2023) "Analyzing Histone Chaperone ANTI-SILENCING FACTOR-1A and HISTONE REPRESSOR A Interactions and Their Roles in Arabidopsis thaliana Development via CRISPR/Cas9 Mutagenesis," SACAD: John Heinrichs Scholarly and Creative Activity Days: Vol. 2023, Article 125.
DOI: 10.58809/QRUG3218
Available at: https://scholars.fhsu.edu/sacad/vol2023/iss2023/125