RNA Interference of the Unfolded Protein Response in Acyrthosiphon pisum

Authors

Jared Ridder, Fort Hays State UniversityFollow
James Balthazor, Fort Hays State UniversityFollow

Award Level

2nd Place - Empirical Graduate

Classification

Empirical Graduate

Abstract

Aphid species are distributed worldwide and are persistent agricultural pests. Previous studies have demonstrated the efficacy of dsRNA as a tool to cause selective death in target organisms through RNA interference ( Balthazor , J. R., et al 2018). Whole RNA was isolated from adult A. pisum and used to synthesize cDNA. Synthesized cDNA was then used as a template to produce dsRNA for use in feeding studies with pea aphids . dsRNAs complementary to target mRNAs were fed to adult aphids and survivorship was assessed. Concentrations of target mRNAs were assessed by quantitative real time PCR (qPCR)

Department/Program

Biology

Submission Type

in-person poster

DOI

10.58809/NZJE1762

Date

4-3-2019

Rights

Copyright the Author(s)

Comments

For questions contact ScholarsRepository@fhsu.edu

Recommended Citation

Ridder, Jared and Balthazor, James (2019) "RNA Interference of the Unfolded Protein Response in Acyrthosiphon pisum," SACAD: John Heinrichs Scholarly and Creative Activity Days: Vol. 2019, Article 4.
DOI: 10.58809/NZJE1762
Available at: https://scholars.fhsu.edu/sacad/vol2019/iss2019/4