2nd Place - Empirical Graduate
Aphid species are distributed worldwide and are persistent agricultural pests. Previous studies have demonstrated the efficacy of dsRNA as a tool to cause selective death in target organisms through RNA interference ( Balthazor , J. R., et al 2018). Whole RNA was isolated from adult A. pisum and used to synthesize cDNA. Synthesized cDNA was then used as a template to produce dsRNA for use in feeding studies with pea aphids . dsRNAs complementary to target mRNAs were fed to adult aphids and survivorship was assessed. Concentrations of target mRNAs were assessed by quantitative real time PCR (qPCR)
Copyright the Author(s)
Ridder, Jared and Balthazor, James
"RNA Interference of the Unfolded Protein Response in Acyrthosiphon pisum,"
SACAD: John Heinrichs Scholarly and Creative Activity Days: Vol. 2019, Article 4.
Available at: https://scholars.fhsu.edu/sacad/vol2019/iss2019/4