2nd Place - Empirical - Graduate
Aphid species are distributed worldwide and are persistent agricultural pests. Previous studies have demonstrated the efficacy of dsRNA as a tool to cause selective death in target organisms through RNA interference ( Balthazor , J. R., et al 2018). Whole RNA was isolated from adult A. pisum and used to synthesize cDNA. Synthesized cDNA was then used as a template to produce dsRNA for use in feeding studies with pea aphids . dsRNAs complementary to target mRNAs were fed to adult aphids and survivorship was assessed. Concentrations of target mRNAs were assessed by quantitative real time PCR (qPCR)
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Ridder, Jared and Balthazor, James, "RNA Interference of the Unfolded Protein Response in Acyrthosiphon pisum" (2019). 2019 SACAD Winners. 8.