Plant-Soil Microbiome Feedback Impacts on Native and Non-native Grasses Throughout Kansas

Scout Harrison, Fort Hays State University

Description

Soil samples will be taken from several locations across the precipitation gradient in Kansas. The core sites will consist of Fort Hays State University, Konza Prairie, and Welda Prairie. We will also be collecting samples at satellite sites which may include Tribune, The Land Institute, the Land Institute-East, the KU Field Station, Smoky Valley Ranch, and private lands. Samples will be collected beginning in August of 2018. Soil samples will be collected from below monospecific stands of the two invasive species yellow bluestem (Bothriochloa ishaemum) and Caucasian bluestem (Bothriochloa bladhii), as well as below the two native species little bluestem (Schizachyrium scoparium), and big bluestem (Andropogon gerardii). Genetic data will be extracted from non-animal soil microbial communities. This will include small subunit ribosomal (SSU) ITS region of DNA for fungi, bacterial 16S rRNA, and algal chloroplast DNA. Data will be analyzed for variation using an ANOVA. Variation of richness and community structure will be analyzed between the grass species and across the precipitation gradient.

 

Plant-Soil Microbiome Feedback Impacts on Native and Non-native Grasses Throughout Kansas

Soil samples will be taken from several locations across the precipitation gradient in Kansas. The core sites will consist of Fort Hays State University, Konza Prairie, and Welda Prairie. We will also be collecting samples at satellite sites which may include Tribune, The Land Institute, the Land Institute-East, the KU Field Station, Smoky Valley Ranch, and private lands. Samples will be collected beginning in August of 2018. Soil samples will be collected from below monospecific stands of the two invasive species yellow bluestem (Bothriochloa ishaemum) and Caucasian bluestem (Bothriochloa bladhii), as well as below the two native species little bluestem (Schizachyrium scoparium), and big bluestem (Andropogon gerardii). Genetic data will be extracted from non-animal soil microbial communities. This will include small subunit ribosomal (SSU) ITS region of DNA for fungi, bacterial 16S rRNA, and algal chloroplast DNA. Data will be analyzed for variation using an ANOVA. Variation of richness and community structure will be analyzed between the grass species and across the precipitation gradient.