RNA Interference of the Unfolded Protein Response in Acyrthosiphon pisum

Jared Ridder, Fort Hays State University

This poster tied for first place in its category.

Description

Aphid species are distributed worldwide and are persistent agricultural pests. Previous studies have demonstrated the efficacy of dsRNA as a tool to cause selective death in target organisms through RNA interference (Balthazor, J. 2018). Whole RNA was isolated from adult A. pisum and used to synthesize cDNA. Synthesized cDNA was then used as a template to produce dsRNA for use in feeding studies with A. pisum.

 

RNA Interference of the Unfolded Protein Response in Acyrthosiphon pisum

Aphid species are distributed worldwide and are persistent agricultural pests. Previous studies have demonstrated the efficacy of dsRNA as a tool to cause selective death in target organisms through RNA interference (Balthazor, J. 2018). Whole RNA was isolated from adult A. pisum and used to synthesize cDNA. Synthesized cDNA was then used as a template to produce dsRNA for use in feeding studies with A. pisum.