Maintaining and axenizing live cyanobacterial cultures for bacterial genome sequencing

Harmful cyanobacterial blooms are an
increasing concern in western Kansas, yet the
bloom-forming species in this region remain
poorly characterized. In this study, we
established and maintained axenic
cyanobacterial cultures to support future
genomic and ecological investigations. By
ensuring low contamination levels and stable
growth conditions, this work provides a
foundation for downstream analyses of bloom-
forming cyanobacteria. It contributes to a better
understanding of freshwater ecosystem health
in western Kansas.